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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Cystatin-A (CSTA)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Cystatin-A (CSTA)品名简称:
Recombinant Human CSTA protein货号:
CSB-EP006099HU规格:
20μg价格:
1536LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
CSTAUniprot No.:
P01040别名:
(Cystatin-AS)(Stefin-A)种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
38.3 kDa表达区域:
2-98aa氨基酸序列:
IPGGLSEAKPATPEIQEIVDKVKPQLEEKTNETYGKLEAVQYKTQVVAGTNYYIKVRAGDNKYMHLKVFKSLPGQNEDLVLTGYQVDKNKDDELTGF蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
This is an intracellular thiol proteinase inhibitor. Has an important role in desmosome-mediated cell-cell adhesion in the lower levels of the epidermis.功能2:
/文献:
"Mutations in CSTA, encoding Cystatin A, underlie exfoliative ichthyosis and reveal a role for this protease inhibitor in cell-cell adhesion." Blaydon D.C., Nitoiu D., Eckl K.M., Cabral R.M., Bland P., Hausser I., van Heel D.A., Rajpopat S., Fischer J., Oji V., Zvulunov A., Traupe H., Hennies H.C., Kelsell D.P. Am. J. Hum. Genet. 89:564-571(2011)研究领域:
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文献和实验三句话读懂一篇 CNS:晚饭吃太晚,更易导致肥胖;为什么女性更容易患老年痴呆?
萎缩和骨质流失之间存在关联,但机制不明。 2022 年 10 月 6 日,南方医科大学黄彬团队在 Nature Aging 杂志发表研究论文 Skin chronological aging drives age-related bone loss via secretion of cystatin-A。 该研究表明过早的皮肤老化会导致小鼠的骨质流失,确定 Csta 是一种表皮源性激素,将皮肤衰老与年龄相关的骨丢失关联起来。皮肤 Csta 水平的增强剂可作为治疗老年骨质疏松症的潜在外用药物。 图
一、胱氨酸蛋白酶抵制剂C( Cystatin C)的性质 胱氨酸蛋白酶抵制剂C( Cystatin C)是一种糖基化的低分子量蛋白质,可自由通过肾小球并被近端肾小管的上皮细胞重吸收和代谢,其血中浓度不受炎症、肌肉量、小管分泌等影响,其血清浓度与肾小球滤过率(GFR)密切相关,可作为肾小球滤过功能指标。 二、监测肾小球滤过率功能最敏感的指标 肾小球滤过率是监测肾功能的一个重要指标。尤其对于肾移植的病人,快速准确地掌握肾小球滤过率的的变化是十分重要
体外。③基本上不被肾小管重吸收或排泌,不受肾小管功能变化的影响。肾小球清除率试验能直接、敏感地反映肾功能。临床常用的有内生肌酐清除率和近年来开展的血清半胱氨酸蛋白酶抑制剂C(cystatin C)测定。此外,血清中肌酐、尿素、尿酸含量的变化也在一定程度可反映肾小球的滤过功能。 ㈠ 内生肌酐清除率测定 肌酐(creatinine)是肌肉中磷酸肌酸的代谢产物,健康人在安静状况下肌肉活动产生的肌酐量基本恒定,而且所产生的肌酐从肾小球滤过后,不被肾小管重吸收,近端小管仅排泌少量。因此,测定血清
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