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MMP10/MMP10蛋白Recombinant Human

Stromelysin-2 (MMP10)重组蛋白Matrix metalloproteinase-10 ;MMP-10Transin-2蛋白
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  • ¥1500 - 12060
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  • CSB-YP014657HU
  • 2025年07月15日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Human Stromelysin-2 (MMP10)

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      1mg/100μg/20μg

    规格:1mg产品价格:¥12060.0
    规格:100μg产品价格:¥2820.0
    规格:20μg产品价格:¥1500.0

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    基因名:

    MMP10

    Uniprot No.:

    P09238

    别名:

    Matrix metalloproteinase-10 ;MMP-10Transin-2

    种属:

    Homo sapiens (Human)

    蛋白长度:

    Full Length of Mature Protein

    来源:

    Yeast

    分子量:

    45 kDa

    表达区域:

    99-476aa

    氨基酸序列:

    FSSFPGMPKWRKTHLTYRIVNYTPDLPRDAVDSAIEKALKVWEEVTPLTFSRLYEGEADIMISFAVKEHGDFYSFDGPGHSLAHAYPPGPGLYGDIHFDDDEKWTEDASGTNLFLVAAHELGHSLGLFHSANTEALMYPLYNSFTELAQFRLSQDDVNGIQSLYGPPPASTEEPLVPTKSVPSGSEMPAKCDPALSFDAISTLRGEYLFFKDRYFWRRSHWNPEPEFHLISAFWPSLPSYLDAAYEVNSRDTVFIFKGNEFWAIRGNEVQAGYPRGIHTLGFPPTIRKIDAAVSDKEKKKTYFFAADKYWRFDENSQSMEQGFPRLIADDFPGVEPKVDAVLQAFGFFYFFSG_S_SQFEFDPNARMVTHILKSNSWLHC

    蛋白标签:

    N-terminal 6xHis-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    货期:

    25-35 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能:

    Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

    内毒素:

    Not test.

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    LC-MS Image Description/Western Blot:

    /

    Product types:

    Developed Protein

    Biological_Activity:

    /

    Research Areas:

    Cancer

    Reconstitution:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    Reference:

    The collagenase gene family in humans consists of at least four members.Muller D., Quantin B., Gesnel M.-C., Millon-Collard R., Abecassis J., Breathnach R.Biochem. J. 253:187-192(1988)

    Function:

    Can degrade fibronectin, gelatins of type I, III, IV, and V; weakly collagens III, IV, and V. Activates procollagenase.

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    图标文献和实验
    该产品被引用文献
    The collagenase gene family in humans consists of at least four members.Muller D., Quantin B., Gesnel M.-C., Millon-Collard R., Abecassis J., Breathnach R.Biochem. J. 253:187-192(1988)
    相关实验
    • Expression of Recombinant Matrix Metalloproteinases in Escherichia coli

      -3, matrilysin, elastase, collagenase-1, collagenase-3, neutrophil collagenase, and membrane type-1 MMP (1 –20 ). The expression of a truncated form of human stromelysin-1 (SL-1) will be used to illustrate the methods utilized for the expression of a matrix

    • Measurement of Matrix Metalloproteinase Activities in the Medium of Cultured Synoviocytes Using Zymography

      Human synovial cells in culture, particularly those obtained from patients with rheumatoid arthritis, secrete a number of matrix metalloproteinases (MMPs) into the culture medium. Enzymes secreted include collagenases (MMP-1, MMP-13

    • 蛋白复性(二)-基因重组蛋白质的体外复性

      X-100或脱氧胆酸钠)和低浓度变性剂(2mol/L尿素或盐酸胍等)洗涤除去脂类和膜蛋白,这一步很重要,否则会导致包涵体溶解和复性的过程中重组蛋白质的降解[6、7、8]。 包涵体的溶解必须用很强的变性剂,如8mol/L尿素、6~8mol/L盐酸胍,通过离子间的相互作用破坏包涵体蛋白间的氢键而增溶蛋白。其中尿素的增溶效果稍差,异氰盐酸胍最强;去污剂,如SDS[7],可以破坏蛋白内的疏水键,可以增溶几乎所有的蛋白,但由于无法彻底去除而不允许用在制药行业中;酸,如70%甲酸[9],可以破坏蛋白

    图标技术资料

    暂无技术资料 索取技术资料

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    文献支持
    MMP10/MMP10蛋白Recombinant Human Stromelysin-2 (MMP10)重组蛋白Matrix metalloproteinase-10 ;MMP-10Transin-2蛋白
    ¥1500 - 12060