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细胞污染识别与处理全攻略:5 种常见类型+关键误区Cytoscape 教程来了!快速实现顶刊同款通路网络图五大应用案例:Mustang Q 膜层析应用全解析1 个小工具,一次性搞定流程图、质粒图谱和信号通路图- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Myoglobin (Mb)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
1mg/100μg/20μg
| 规格: | 1mg | 产品价格: | ¥14844.0 |
|---|---|---|---|
| 规格: | 100μg | 产品价格: | ¥3456.0 |
| 规格: | 20μg | 产品价格: | ¥1836.0 |
纯度:
Greater than 90% as determined by SDS-PAGE.基因名:
MbUniprot No.:
P04247别名:
Mb; Myoglobin种属:
Mus musculus (Mouse)蛋白长度:
Full Length of Mature Protein来源:
Yeast分子量:
20.4 kDa表达区域:
2-154aa氨基酸序列:
GLSDGEWQLVLNVWGKVEADLAGHGQEVLIGLFKTHPETLDKFDKFKNLKSEEDMKGSEDLKKHGCTVLTALGTILKKKGQHAAEIQPLAQSHATKHKIPVKYLEFISEIIIEVLKKRHSGDFGADAQGAMSKALELFRNDIAAKYKELGFQG蛋白标签:
N-terminal 6xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能:
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.内毒素:
Not test.SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.LC-MS Image Description/Western Blot:
/Product types:
In Stock ProteinBiological_Activity:
/Research Areas:
CardiovascularReconstitution:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Reference:
"The mouse myoglobin gene. Characterisation and sequence comparison with other mammalian myoglobin genes." Blanchetot A., Price M., Jeffreys A.J. Eur. J. Biochem. 159:469-474(1986)Function:
Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验CK-MB可以比CK高, 国内及国外的很多报道有上述现象,只要CKMB的升高不是由于溶血造成的,CKMB >CK,这样的病例95%是O型或B型血的癌症患者,原因是部分癌症病人免疫系统混乱,其中的一些免疫球蛋白充当的辅酶的作用。 在临床检测中,有时会出现CKMB检测结果比CK还要高的情况,会给临床医生造成一定的困扰。首先介绍免疫抑制法检测CK-MB活性的原理:应用单克隆抗体与CK中的M 亚基结合进而抑制肌酸激酶同工酶CK-MM 以及CK-MB中的M 亚基的活性后,再通
MDA-MB-231源自一名51岁转移性乳腺腺癌女性患者的胸腔积液,ER/PR不表达、HER2无扩增/不表达,常作为三阴性乳腺癌(TNBC)及侵袭转移研究的经典模型。该细胞贴壁生长,呈上皮样,但实际培养中常见梭形、间质样、EMT相关表型,亚型上多接近claudin-low或basal B特征。培养时要避免过密导致老化,胰酶消化时间建议短、且终止消化要充分,以减少对后续贴壁与活力的影响。培养中偶见少量漂浮细胞。 一、细胞复苏 1.【准备工作】水浴锅提前预热至37℃;准备完全培养基:DMEM
://www.uniprot.org/uniprot/P68871 见网址,只在红细胞中表达! 我把β globin理解为血红蛋白中β链珠蛋白,显然只在红细胞中表达,有携氧功能。 补充一下珠蛋白的内容: 珠蛋白是一类能够通过铁卟啉环可逆性结合氧的呼吸性蛋白质,目前在脊椎动物体内已有4种类型的珠蛋白被鉴定和正式命名:血红蛋白(haemoglobin,Hb),肌红蛋白(myoglobin,Mb),脑红蛋白(neuroglobin,Ngb)和细胞珠蛋白
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