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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Bovine Alpha-crystallin B chain (CRYAB)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Bovine Alpha-crystallin B chain (CRYAB)品名简称:
Recombinant Bovine CRYAB protein货号:
CSB-EP006008BO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
CRYABUniprot No.:
P02510别名:
(Alpha(B)-crystallin)种属:
Bos taurus (Bovine)蛋白长度:
Full Length来源:
E.coli分子量:
27.5 kDa表达区域:
1-175aa氨基酸序列:
MDIAIHHPWIRRPFFPFHSPSRLFDQFFGEHLLESDLFPASTSLSPFYLRPPSFLRAPSWIDTGLSEMRLEKDRFSVNLDVKHFSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLAITSSLSSDGVLTVNGPRKQASGPERTIPITREEKPAVTAAPKK蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
May contribute to the transparency and refractive index of the lens. Has chaperone-like activity, preventing aggregation of various proteins under a wide range of stress conditions. In lens epithelial cells, stabilizes the ATP6V1A protein, preventing its degradation by the proteasome.功能2:
/文献:
"Identification of the posttranslational modifications of bovine lens alpha B-crystallins by mass spectrometry." Smith J.B., Sun Y., Smith D.L., Green B. Protein Sci. 1:601-608(1992)研究领域:
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文献和实验【求助】哪里能买到含有人干扰素alpha-2b cDNA序列的质粒pBV889
baiguo861029 各位前辈好 本人是个新手,听很多师兄师姐说丁香园很好,这儿的人都很热情帮助他人。我现在研究生在读,因为课题需要,急需购买这个质粒pBV889,试了好多方法都没找到。这个质粒含有人干扰素alpha-2b编码序列,即cDNA序列。非常希望知道的前辈给予我帮助,非常感谢!! woxingwosu 用google搜索了一下,发现很多文章都有,国内发表的也很多。其实你或者你老板可以写信去要一下就方便多了,还不用
In situ hybridisation to alpha satellite sequences (chromosome specific)
Alpha satellite sequences, whilst highly repetitive, are specific to each individual chromosome. These sequences flank the centromeres and can present a target measured in megabases. In this protocol a biotin or digoxigenin labelled DNA
夹心法ELISA检测人α2a,α2b干扰素(α2a,α2b)
重组人α2a/α2b干扰素(rHuIFN-α2a、rHuIFN-α2b)生产流程中不可缺少的一环是产品的质量监控,通常采用的是HEp-2/VSV或Wish/VSV系统检测干扰素的生物活性。此系统常由于VSV或细胞的影响而不够稳定,且检测周期较长。我们用自制的2种针对rHuIFN-α2a和rHuIFN-α2b的单克隆抗体(McAb)建立了定量检测rHuIFN-&alpha
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