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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Interleukin-1 alpha (IL1A)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Interleukin-1 alpha (IL1A)品名简称:
Recombinant Human IL1A protein货号:
CSB-EP011613HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
IL1AUniprot No.:
P01583别名:
Hematopoietin-1种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
22 kDa表达区域:
113-271aa氨基酸序列:
SAPFSFLSNVKYNFMRIIKYEFILNDALNQSIIRANDQYLTAAALHNLDEAVKFDMGAYKSSKDDAKITVILRISKTQLYVTAQDEDQPVLLKEMPEIPKTITGSETNLLFFWETHGTKNYFTSVAHPNLFIATKQDYWVCLAGGPPSITDFQILENQA蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.功能2:
Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.文献:
Cloning, sequence and expression of two distinct human interleukin-1 complementary DNAs.March C.J., Mosley B., Larsen A., Cerretti D.P., Braedt G., Price V., Gillis S., Henney C.S., Kronheim S.R., Grabstein K., Conlon P.J., Hopp T.P., Cosman D.Nature 315:641-647(1985)研究领域:
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文献和实验为细胞培养上清,因该类样本干扰因素 较多,如:细胞状态、细胞数量、采样时间等,所以可能存在检测不出的情况。6. 某些天然蛋白或重组蛋白,包括原核及真核重组蛋白,可能因为与本产品所使用的检测抗体及捕获抗体不匹配,而不被检测出。7. 建议使用新鲜样本,保存时间过长可能会存在蛋白降解或变性导致实验结果偏差。操作步骤1. 加样:分别设标准孔、待测样品孔、空白孔。设标准孔 7 孔,依次加入 100μL 不同浓度的标准品 (见试剂准备 2)。空白孔加 100μL(见试剂准备第二步最后一管),余孔加待测样品
的靶点分布呈现出明显集中的特点,主要集中在少数几个关键的激酶家族上[19]。 表3. 获批激酶抑制剂的靶点分布[19]。 总的来说,已获批激酶抑制剂的靶点分布高度集中于驱动癌症和自身免疫性疾病的关键信号通路上,尤其是酪氨酸激酶家族。 MCE 相关产品 一、主要激酶家族的重组蛋白:含常见突变体如 EGFR L858R/T790M/C797S(www
上包含的基因可编码上述信号通路中的粘附分子、受体、配体、接头蛋白、调控因子、转录因子、激酶及下游效应蛋白。信号转导在免疫细胞激活、增殖、分化和凋亡中扮演了重要的角色,而这些生物学过程也与许多重要的病理及生理免疫反应有关,如:抗病毒反应、自身免疫、过敏症、炎症,及免疫缺陷。 基因列表(Functional Gene Grouping): AKT and PI3K Family Members and Their Regulators: AKT1, AKT2, AKT3, APPL, BTK
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