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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Mouse Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 (Ndst2), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Mouse Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 2 (Ndst2), partial品名简称:
Recombinant Mouse Ndst2 protein, partial货号:
CSB-EP015615MO规格:
20μg价格:
2328LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 85% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
Ndst2Uniprot No.:
P52850别名:
(Glucosaminyl N-deacetylase/N-sulfotransferase 2)(NDST-2)(Mndns)(N-heparan sulfate sulfotransferase 2)(N-HSST 2)种属:
Mus musculus (Mouse)蛋白长度:
Partial来源:
E.coli分子量:
40.3 kDa表达区域:
598-883aa氨基酸序列:
KTCDRLPKFLIVGPQKTGTTAIHFFLSLHPAVTSSFPSPSTFEEIQFFNGPNYHKGIDWYMDFFPVPSNASTDFLFEKSATYFDSEVVPRRGAALLPRAKIITVLINPADRAYSWYQHQRAHGDPIALNYTFYQVISASSQAPLLLRSLQNRCLVPGYYSTHLQRWLTYYPSGQLLIMDGQELRVNPAASMEIIQKFLGITPFLNYTRTLRFDEDKGFWCQGLEGGKTRCLGRSKGRRYPDMDMESRLFLTDFFRNHNLELSKLLSRLGQPAPLWLREELQHSSVG蛋白标签:
N-terminal 10xHis-tagged and C-terminal Myc-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Plays a role in determining the extent and pattern of sulfation of heparan sulfate. Required for the exosomal release of SDCBP, CD63 and syndecan.功能2:
/文献:
"Abnormal mast cells in mice deficient in a heparin-synthesizing enzyme." Forsberg E., Pejler G., Ringvall M., Lunderius C., Tomasini-Johansson B., Kusche-Gullberg M., Eriksson I., Ledin J., Hellman L., Kjellen L. Nature 400:773-776(1999)研究领域:
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文献和实验,并且 EST12-RACK1 诱导的细胞焦亡在 M.tb 诱导的免疫中起关键作用。 研究内容: 1、RD 区编码蛋白筛选:对 40 种 RD 区(RD1-3、11-14)重组蛋白进行筛选(巨噬细胞焦磷酸相关蛋白),通过 LDH 释放实验细胞毒性分析,发现 EST12 对巨噬细胞有显著焦解作用。 2、菌株构建:构建 M. tb H37Rv ΔEST12(Rv1579c 敲除菌株)、BCG-EST12(Rv1579c 过表达菌株)、M. smeg–EST12(Rv1579c 过表达菌株)。 3、小鼠
) 。 ( 3 ) 384 孔微量滴定板(Gentix,Christchurch,UK ) 。 ( 4 ) 96 孔深孔板:每孔容积 2 ml 的 96 孔微滴定板(Qiagen,Hilden,Germany) 。 ( 5 ) 96 针复制器(Nunc,Wiesbaden,Germany) 。 ( 6 ) 4 X SB 培养基:48 g/L 胰化蛋白胨,96 g/L 酵母提取物,0.8% ( V/V)甘油,120°C 高压灭菌 20 min。 ( 7 ) 20 X PP 缓冲液(磷酸钾盐缓冲
7 African green monkey SV40 transformed kidney 非洲绿猴SV40转化肾细胞 ● CA77 Rat Medulary thyroid tumor 大鼠髓质甲状腺肿瘤细胞 ● EB-3 Human Burkitt’s lymphoma 人伯基特氏淋巴瘤细胞 ● FM3A Mouse Breast cancer 小鼠乳腺癌细胞 ● GC, GH3 Rat Pituitary 大鼠垂体脑细胞 ● HEL, N-10
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