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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Purified nontoxic mutant Diphtheria toxin CRM197
- 亚型:
IgG
- 形态:
Liquid
- 保存条件:
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性:
Polyclonal
- 标记物:
Unconjugated
- 适应物种:
Corynebacterium diphtheriae
- 保质期:
12 months from the shipping date of the product.
- 抗原来源:
Corynebacterium diphtheriae
- 目录编号:
GTX00757
- 级别:
Primary Antibodies
- 库存:
Available
- 供应商:
GeneTex
- 宿主:
Rabbit
- 应用范围:
WB, IP, Dot, ELISA
- 靶点:
This antibody could detect 1 ng of the toxin by ELISA and Dot blot.
- 抗体英文名:
Diphtheria Toxin antibody
- 抗体名:
Diphtheria Toxin 抗体
- 规格:
100 μl
WB analysis of culture medium of Corynebacterium Diphtheria using GTX00757 Diphtheria Toxin antibody.
Lane 1 : Crude culture medium of Corynebacterium Diphtheria
Lane 2 : The two-fold diluted culture medium
Lane 3 : Purified Diphtheria CRM197 mutant protein (10ng)
Lane 4 : Purified wide type Diphtheria toxin (200ng)
Dilution : 1:2000
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文献和实验The fusion protein toxins that have been described are generally composed of the catalytic and transmembrane domains of a bacterial toxin (e.g., diphtheria toxin [DT] or Pseudomonas exotoxin A) to which a polypeptide hormone, growth factor
Preparation of Cytotoxic Antibody-Toxin Conjugates
) that inactivates eukaryotic ribosomes by a specific irreversible covalent modification of the ribosomal RNA (2 ). The B chain binds to cell surface galactose-containing oligosaccharide residues. Following receptor-mediated endocytosis of toxin bound to the cell
Immunoaffinity Purification and Quantification of Antibody-Toxin Conjugates
Cytotoxic antibody-toxin conjugates made using antibodies and ribs some-inactivating proteins (RIPs) are prepared using chemical crosslinking methods (1 ,2 and this vol., Chapter 31 ). Gel permeation chromatography is used as a first step
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