- ￥5600
- GeneTex
- 美国
- GTX00688
- 2025年07月13日
- WB, ICC/IF, FACS
- Mouse
- Human, Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
A synthetic peptide corresponding to human RBP2, amino acids 1416-1434.
- 亚型：
IgG2a
- 形态：
Liquid
- 保存条件：
Store as concentrated solution. Centrifuge briefly prior to opening vial. For short-term storage (1-2 weeks), store at 4ºC. For long-term storage, aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
- 克隆性：
Monoclonal
- 标记物：
Unconjugated
- 适应物种：
Human, Mouse
- 保质期：
12 months from the shipping date of the product.
- 抗原来源：
Human
- 目录编号：
GTX00688
- 级别：
Primary Antibodies
- 库存：
Available
- 供应商：
GeneTex
- 宿主：
Mouse
- 应用范围：
WB, ICC/IF, FACS
- 浓度：
1 mg/ml (Please refer to the vial label for the specific concentration.)
- 靶点：
KDM5A / Jarid1A / RBBP2
- 抗体英文名：
KDM5A / Jarid1A / RBBP2 antibody [18E8]
- 抗体名：
KDM5A / Jarid1A / RBBP2 抗体 [18E8]
- 规格：
100 μg
WB analysis of various samples using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8].
Left panel : SDS-PAGE with CBB staining
Right panel : Primary antibody
Lane 1 : HeLa cells transfected control siRNA
Lane 2 : HeLa cells transfected RBP2 siRNA
Lane 3 : MCF-7 whole cell lysate
Lane 4 : U2OS whole cell lysate
Lane 5 : NIH3T3 whole cell lysate
Lane 6 : J1 (mouse ES cells) whole cell lysate
ICC/IF analysis of HeLa cells using GTX00688 KDM5A / Jarid1A / RBBP2 antibody [18E8].
Dilution : 1:2000
Fixation : 4% PFA, overnight
Permeabilization : 0.25% Triton X-100 in PBS for 10 min
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文献和实验Nishibuchi G et al., J Biol Chem.? 2014 (PMID:25190814)
ChIP protocol for X. laevis Lens1/FoxE3 enhancer
to agarose beads in chromatin samples prior to antibody reaction. (17) Pellet the agarose beads by brief centrifugation (3000-5000 x g (ex. 6600 rpm at Eppendorf table top centrifuge) for 1 min)). (18) Collect supernatants into new tubes. Remove
Simultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human Lymphocytes
measures the frequency of t(14;18) translocations that result in the dysfunctional regulation of the antiapoptotic gene BCL-2 . The other assay measures the frequency of a deletion caused by illegitimate V(D)J recombination in the X-linked HPRT gene.
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