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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Translation initiation factor eIF-2B subunit beta (EIF2B2)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Translation initiation factor eIF-2B subunit beta (EIF2B2)品名简称:
Recombinant Human EIF2B2 protein货号:
CSB-EP007515HU规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
EIF2B2Uniprot No.:
P49770别名:
S20I15 S20III15 eIF-2B GDP-GTP exchange factor subunit beta种属:
Homo sapiens (Human)蛋白长度:
Full Length来源:
E.coli分子量:
66 kDa表达区域:
1-351aa氨基酸序列:
MPGSAAKGSELSERIESFVETLKRGGGPRSSEEMARETLGLLRQIITDHRWSNAGELMELIRREGRRMTAAQPSETTVGNMVRRVLKIIREEYGRLHGRSDESDQQESLHKLLTSGGLNEDFSFHYAQLQSNIIEAINELLVELEGTMENIAAQALEHIHSNEVIMTIGFSRTVEAFLKEAARKRKFHVIVAECAPFCQGHEMAVNLSKAGIETTVMTDAAIFAVMSRVNKVIIGTKTILANGALRAVTGTHTLALAAKHHSTPLIVCAPMFKLSPQFPNEEDSFHKFVAPEEVLPFTEGDILEKVSVHCPVFDYVPPELITLFISNIGGNAPSYIYRLMSELYHPDDHVL蛋白标签:
N-terminal GST-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.功能2:
Catalyzes the exchange of eukaryotic initiation factor 2-bound GDP for GTP.文献:
"N-terminal acetylome analyses and functional insights of the N-terminal acetyltransferase NatB." Van Damme P., Lasa M., Polevoda B., Gazquez C., Elosegui-Artola A., Kim D.S., De Juan-Pardo E., Demeyer K., Hole K., Larrea E., Timmerman E., Prieto J., Arnesen T., Sherman F., Gevaert K., Aldabe R. Proc. Natl. Acad. Sci. U.S.A. 109:12449-12454(2012)研究领域:
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文献和实验帮助将抑制因子tRNA交付到核糖体的翻译因子eIF2利用GTP水解的能量来发挥功能。另一个因子eIF5已知在当eIF2与抑制因子tRNA和核糖体结合时会加快eIF2的GTP酶活性。在这项研究中,研究人员确定了eIF5的另外两个作用。 一个涉及在eIF2上稳定GDP(GTP水解的产物);另一个涉及其通过磷酸化的eIF2来发挥作用、以抑制鸟甙酸交换因子eIF2B。这些结果澄清了我们对翻译抑制怎样被调控的认识。 eIF5 has GDI activity necessary
[资源] 所有的看家基因(housekeeping genes)列表+引物设计服务
initiation factor 3, subunit 2 beta, 36kDa (EIF3S2), mRNA 622 NM_001360 Homo sapiens 7-dehydrocholesterol reductase (DHCR7), mRNA 653 NM_001344 Homo sapiens defender against cell death 1 (DAD1), mRNA 812 NM_001914 Homo sapiens cytochrome b-5 (CYB5
所有的看家基因(housekeeping genes)的列表+引物设计服务
(H2AV), transcript variant 1, mRNA 1070 NM_012401 Homo sapiens plexin B2 (PLXNB2), mRNA 929 NM_007262 Homo sapiens RNA-binding protein regulatory subunit (DJ-1), mRNA 1514 NM
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