GAPDH|GAPDH蛋白|GAPDH兔蛋白|GAPDH R

【信息】Rabbit anti-GAPDH(SF-PA005)（兔抗 GAPDH 多克隆 IgG 抗体）招代理

GAPDH多克隆IgG抗体源自重组人GAPDH蛋白 C 端片段免疫新西兰大白兔。 特异性 兔抗 GAPDH 多克隆 IgG 抗体推荐用于人、 大鼠、小鼠及其它哺乳动物 GAPDH 蛋白检测，特异性?选?/p> 纯化 分离抗血清并亲和层析纯化。 应用 免疫组化 1:100-1:400 免疫荧光 1:100-1:400 Western Blot 1:400-1:800 ELISA 1:2,000-1:8,000 最优稀释比例依不同实验具体情况而定。 保存

用RNaseIII制备siRNA库来诱发RNAi

. A sample was run on a 15% non-denaturing acrylamide gel for visualization (2A). HeLa cells were transfected with 100 nM of the 12-15 bp RNase III generated GAPDH siRNA s or a 21 bp chemically synthesized GAPDH siRNA . GAPDH protein levels were monitored

用RNase III制备siRNA库来诱发RNAi

chemically synthesized GAPDH siRNA . GAPDH protein levels were monitored by immunofluorescence 48 hours after transfection (2B) and the resulting images were quantitated (2C). 基因沉默的专一性 进一步的实验来验证这种方法制备的siRNA 库抑制靶基因表达的专一性：用RNase III 制备的针对GAPDH 的siRNA 库转染Hela