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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Granzyme K (GZMK)
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Granzyme K (GZMK)品名简称:
Recombinant Human GZMK protein货号:
CSB-EP010084HU规格:
20μg价格:
1836LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
GZMKUniprot No.:
P49863别名:
(Fragmentin-3)(Granzyme-3)(NK-tryptase-2)(NK-Tryp-2)种属:
Homo sapiens (Human)蛋白长度:
Full Length of Mature Protein来源:
E.coli分子量:
29.9 kDa表达区域:
27-264aa氨基酸序列:
IIGGKEVSPHSRPFMASIQYGGHHVCGGVLIDPQWVLTAAHCQYRFTKGQSPTVVLGAHSLSKNEASKQTLEIKKFIPFSRVTSDPQSNDIMLVKLQTAAKLNKHVKMLHIRSKTSLRSGTKCKVTGWGATDPDSLRPSDTLREVTVTVLSRKLCNSQSYYNGDPFITKDMVCAGDAKGQKDSCKGDSGGPLICKGVFHAIVSGGHECGVATKPGIYTLLTKKYQTWIKSNLVPPHTN蛋白标签:
N-terminal 6xHis-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
13-23 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
/功能2:
/文献:
"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004)研究领域:
Cell Biology请点击“在线询价”找客服索取产品说明书及引用文献目录,产品实时更新参数、价格信息、更多文献信息可以查看cusabio官网
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文献和实验Protocol for the Clonal Analysis of NK Cell Effector Functions by Multi-parameter Flow Cytometry
Natural killer (NK) cells provide a first line of defense against viral infections and prepare the ground for subsequent action of virus-specific T cells in a concerted way. Human NK cells use a sophisticated system of inhibitory
细胞,将 T 细胞区分为 CD4+CD8-的辅助性T细胞(T helper cells,TH)和 CD4-CD8+ 的杀伤性T细胞(Cytotoxic T Cells, CTL)。辅助性 T 细胞在免疫应答中,主要通过分泌细胞因子的方式,调控其它免疫细胞的活化;而杀伤性 T 细胞则可以通过细胞毒作用直接对感染或者转化的细胞进行杀伤。 NK 细胞又被称为大颗粒淋巴细胞,其细胞质中存在大量颗粒状的囊泡,储存颗粒酶 B(Granzyme B)等杀伤性免疫效应分子。NK 细胞的功能与 CTL 类似,主要是识别
Fluorogenic Substrates as Detectors of Caspase Activity During Natural Killer Cell-Induced Apoptosis
by the secretory pathway (3 ), whereas granzyme B and CD95 induce early DNA fragmentation by activation of multiple cysteine proteases of the caspase cascade, including caspase-8 (FLICE) (4 ) and caspase-3 (CPP32) (5 ). The exact mechanisms governing NK-induced
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