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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 保质期:
12个月
- 英文名:
Recombinant Human Lon protease homolog, mitochondrial (LONP1), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
10ug/20ug/50ug/100ug/200ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥69.0 |
|---|---|---|---|
| 规格: | 20ug | 产品价格: | ¥139.0 |
| 规格: | 50ug | 产品价格: | ¥209.0 |
| 规格: | 100ug | 产品价格: | ¥349.0 |
| 规格: | 200ug | 产品价格: | ¥419.0 |
| 规格: | 500ug | 产品价格: | ¥559.0 |
| 规格: | 1mg | 产品价格: | ¥699.0 |
Research Areas
CancerUniprot ID
P36776Gene Names
LONP1Alternative Name(s)
(LONHs)(Lon protease-like protein)(LONP)(Mitochondrial ATP-dependent protease Lon)(Serine protease 15)Lead Time
3-7 business daysOrganism
Homo sapiens (Human)Source
E.coliExpression Region
124-368aaProtein Length
PartialTag Info
N-terminal 10xHis-tagged and C-terminal Myc-taggedTarget Protein Sequence
LPLIAITRNPVFPRFIKIIEVKNKKLVELLRRKVRLAQPYVGVFLKRDDSNESDVVESLDEIYHTGTFAQIHEMQDLGDKLRMIVMGHRRVHISRQLEVEPEEPEAENKHKPRRKSKRGKKEAEDELSARHPAELAMEPTPELPAEVLMVEVENVVHEDFQVTEEVKALTAEIVKTIRDIIALNPLYRESVLQMMQAGQRVVDNPIYLSDMGAALTGAESHELQDVLEETNIPKRLYKALSLLKKMW
35.6 kDaPurity
Greater than 90% as determined by SDS-PAGE.Form
Lyophilized powder.If the size you ordered does not have lyophilized powder in stock, we will provide it in liquid formBuffer
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose.Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
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文献和实验三句话读懂一篇 CNS:越漂亮越健康?免疫力竟与颜值有关;肠道菌群失调或引发焦虑抑郁
Communications 杂志发表研究论文 Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength。 该研究利用多个 LONP1 缺失的小鼠模型以及体外原代肌肉细胞培养系统展开探索,发现 LONP1 依赖的线粒体蛋白质稳态控制对肌肉含量和功能维持至关重要,揭示了肌肉废用过程中线粒体蛋白
一、细胞的裂解1. HeLa cells were pelleted using a Sorvall centrifuge at 800 g for 10 min.2. Cells were resuspended in lysis buffer [50 mM Tris-HCl, pH 7.4, 150 mM NaCl, 0.5% NP-40, 5 mM EDTA, 50 mM NaF, 1 mM PMSF and a protease inhibitor(PI) cocktail: 25
by centrifugation for 20 min at 10,000 rcf at 4℃. (Buffer S: sucrose 150 mM, KCl 40 mM, Tris/HCl 25 mM, BSA 1 mg/ml, pH 7.4) (6) Protein concentration in nuclear, cytoplasmic, and mitochondrial fractions were determined according to the Bradford method.
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