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/lon重组蛋白ATP-dependent protease

LaMycoplasma pneumoniae Lon protease(lon)蛋白
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  • ¥2328
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  • CSB-EP305230MLW
  • 2026年01月20日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 保存条件

      We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.

    • 保质期

      Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    • 英文名

      Recombinant Mycoplasma pneumoniae Lon protease (lon), partial

    • 库存

      200

    • 供应商

      武汉华美生物工程有限公司

    • 规格

      20μg


    英文名称:

    Recombinant Mycoplasma pneumoniae Lon protease (lon), partial

    品名简称:

    Recombinant Mycoplasma pneumoniae lon protein, partial

    货号:

    CSB-EP305230MLW

    规格:

    20μg

    价格:

    2328

    LC-MS/Western Blot:

    /

    SDS-PAGE:

    (Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.

    纯度:

    Greater than 90% as determined by SDS-PAGE.

    内毒素:

    Not test

    生物活性:

    /

    基因名:

    lon

    Uniprot No.:

    P78025

    别名:

    ATP-dependent protease La

    种属:

    Mycoplasma pneumoniae (strain ATCC 29342 / M129)

    蛋白长度:

    Partial

    来源:

    E.coli

    分子量:

    39.8 kDa

    表达区域:

    1-206aa

    氨基酸序列:

    MPAVKKPQILVVRNQVIFPYNGFELDVGRERSKKLIKALKNLKTKRLVLVTQKNSDQLNPEFDDIYHCGTLCDIDEIIEVPSEDGKTADYKIKGKGLQRVAITSFSDADLTKYDHHFLNSTLTENKALDKLLERIFPDKEDFAEILDSLNSFLELQELKKLSKVPKDIKRYDIITFKLASLIFKDITLQQAILEENDIEKRLQKII

    蛋白标签:

    N-terminal 6xHis-SUMO-tagged

    产品提供形式:

    Liquid or Lyophilized powder

    缓冲液:

    If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.

    储存条件:

    We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

    保质期:

    The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.

    货期:

    13-23 business days

    注意事项:

    Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.

    功能1:

    ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.

    功能2:

    ATP-dependent serine protease that mediates the selective degradation of mutant and abnormal proteins as well as certain short-lived regulatory proteins. Required for cellular homeostasis and for survival from DNA damage and developmental changes induced by stress. Degrades polypeptides processively to yield small peptide fragments that are 5 to 10 amino acids long. Binds to DNA in a double-stranded, site-specific manner.

    文献:

    "Complete sequence analysis of the genome of the bacterium Mycoplasma pneumoniae."Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.Nucleic Acids Res. 24:4420-4449(1996)

    研究领域:

    Cell Biology

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    相关实验
    • 【转帖】[转贴]外源基因在原核细胞中的表达

      基因多个连接,增加稳定表达。 D、酶学和化学法选择性裂解融合蛋白: GrBr.裂解Met,生产胰岛素; Somatomedin、Acromobacter、Protease I(API)特异裂解Lysine,生产d-hANP。 (二) 从表达产物分泌性来分 1、分泌表达:表达载体或外源基因含信号肽。 常用信号肽:b-lactamase、alkaliphospatase、OmpA、ProteinA 优点:1)防止蛋白酶降解2)有利于纯化3)表达产物N端不含有Met 4)大多为可溶性表达

    • Stress Detection Using Bioluminescent Reporters of the Heat-Shock Response

      , encoding a protein that interacts with Hsp70 in the protein folding pathway, and lon , a protease that degrades unfolded protems. Detection of the heat-shock response has been selected as a useful indicator of biological stress (3 –

    • 【资源】大肠杆菌的表型大全

      of the lacZ gene that allows α complementation of the β-galactosidase gene; required for blue/white selection on XGal plates. Deletes the amino portion of lacZ (aa 11-41). leuB = requires leucine Δlon = deletion of the lon protease malA = cannot metabolize

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    /lon重组蛋白ATP-dependent protease LaMycoplasma pneumoniae Lon protease(lon)蛋白
    ¥2328