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Amino-PEG36-alcohol
MCE 国际站：Amino-PEG36-alcohol
产品活性：Amino-PEG36-alcohol 是一种 PROTAC linker，属于 PEG 类。可用于合成 PROTAC 分子。
研究领域：PROTAC
作用靶点：PROTAC Linkers
In Vitro: PROTACs contain two different ligands connected by a linker; one is a ligand for an E3 ubiquitin ligase and the other is for the target protein. PROTACs exploit the intracellular ubiquitin-proteasome system to selectively degrade target proteins.
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文献和实验-well microtiter plate, and this process is repeated for each of the 48 samples until the entire 96 wells are filled. A solution of polyethylene glycol (PEG) then is added robotically to each well and mixed. The microtiter plate is covered with an acetate plate
Multiwell Transformation Protocol
is enough for 96 transformations, which we rarely do. More typically, we perform 36 haploid and 36 diploid transformations. In this case 100 ml of cells of each is enough. On the day of the transformation: 1. Prepare 1 Liter sterile 100mM LiAcetate
(used for the final plating), add 5 - 10% newborn calf serum. 40% PEG: Aldrich 1000. Made up in serum-free medium (SFM). The stock may be aliquoted and stored at -30°C. Other materials include: 96-well plates, sterile Petri dishes, conical
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