手机验证
发货周期:4-8周
博士德生物
Serum
96T
This Bosterbio ELISA kit is designed to measure the concentration of T3 in human serum by Competitive ELISA (Enzyme-Linked Immunosorbent Assay). The polystyrene micro-well plate in this kit has been pre-coated with an anti-Human T3 antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated T3 and incubated. The T3 found in the sample or standards competes with the biotinylated T3 for limited binding sites on the immobilized anti-Human T3 antibody. Excess unbound biotinylated T3 and sample or standard T3 is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated T3 captured in the well and inversely proportional to the amount of T3 which was contained in the sample or standard.
Product Name | Human Triiodothyronine (T3) ELISA Kit |
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SKU/Catalog Number | EK7010 |
Description | Competitive High Sensitivity ELISA kit for Quantitative Detection of Human T3 96wells/kit, with removable strips. |
Cite This Product | Human Triiodothyronine (T3) ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7010) |
Validated Species | Human |
Application | ELISA *Our Boster Guarantee covers the use of this product in the above tested applications. |
Cross Reactivity | There is no detectable cross-reactivity. |
Pack Size | 96wells/kit, with removable strips. |
Sensitivity | 0.5 ng/mL *Sensitivity, or Lower Limit of Detection (LLD), is the minimum level of target protein the ELISA assay can detect. We measure 20 blank wells and if the O.D. value is 2 standard deviations higher than the blanks' average O.D. the sample can be deemed positive. |
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Assay Range | 0.5-8 ng/mL *This assay range is determined using common samples. For samples with low target protein concentrations, users can adjust the standard curve to extend the lower limit of assay range. |
Sample Type | Serum *The above listed samples are the ones valided with the assay. If you do not see your sample of interest listed, as long as there is enough level of target protein present in the sample, this Picokine? ELISA kit should detect it. **For protocol and tips regarding preparing your sample of interest, please check our ELISA sample preparation guide. |
Storage | Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
Description | Quantity | Volume | Buffers |
Pre-coated 96-well strip microplate | 1 | 8 strips of 12 wells | Rabbit Anti- T3 Antibody, Polystyrene micro-well plate |
Human T3 Standards(S1~S5) | 5 | 1ml | T3 (0.5, 1.0, 2.0, 4.0, 8.0 ng/mL), 100% detoxification serum, 0.1% Proclin-300 |
Avidin-HRP conjugate | 1 | 6ml | Avidin-HRP conjugate, 0.02M PBS, 20% new-born calf serum, 0.01% azophloxine, 0.1% Proclin-300 |
Biotinylated antigen | 1 | 6ml | Biotinylated T3, 0.02M PBS, 20% new-born calf serum, 0.1% Proclin-300 |
Controls | 2 | 1ml | T3, 100% natural protein, 0.1% Proclin-300 |
20X Wash Buffer Concentrate | 1 | 15ml | 0.2M PBS containing 0.5% tween 20 |
Color Developing Reagent A | 1 | 7ml | 11m mol/L Urea hydrogen peroxide |
Color Developing Reagent B | 1 | 7ml | 2m mol/L 3,3'5,5’-Tetramethylbenzidine |
Stop Solution | 1 | 7ml | 2mol/L Sulphuric acid |
Plate Sealers | 2 | Piece |
1. Microplate Reader capable of reading absorbance at 450nm.
2. Automated plate washer (optional)
3. Pipettes and pipette tips capable of precisely dispensing 0.5 μl through 1 ml volumes of aqueous solutions. Multichannel pipettes are recommended for large amount of samples.
4. Deionized or distilledwater.
5. 500ml graduated cylinders.
6. Test tubes for dilution.
Triiodothyronine, also known as T3, is a thyroid hormone. It affects almost every physiological process in the body, including growth and development, metabolism, body temperature, and heart rate. Production of T3 and its prohormone thyroxine (T4) is activated by thyroid-stimulating hormone (TSH), which is released from the anterior pituitary gland.
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[list_product_images] | Human T3 ELISA Kit standard curve[/list_product_images]
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