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发货周期:现货
博士德生物
cell culture supernates, serum and plasma (heparin, EDTA, citrate).
96T
货号 | EK2092 |
---|---|
产品名称 | Human IL4R ELISA Kit |
产品描述 | For quantitative detection of human IL4R in cell culture supernates, serum and plasma (heparin, EDTA, citrate). |
免疫原 | NSO, M26-H232 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <10pg/ml |
检测范围 | 46.9pg/ml-3000pg/ml |
基因名称 | IL4R |
基因全名 | interleukin 4 receptor |
Uniprot ID | P24394 |
指标别名 | Interleukin-4 receptor subunit alpha; IL-4 receptor subunit alpha; IL-4R subunit alpha; IL-4R-alpha; IL-4RA; CD124; Soluble interleukin-4 receptor subunit alpha; Soluble IL-4 receptor subunit alpha; Soluble IL-4R-alpha; sIL4Ralpha/prot; IL-4-binding protein; IL4-BP; IL4R; IL4RA; 582J2.1 |
背景资料 | The interleukin 4 receptor is a type I cytokine receptor. IL4R is its human gene. It is mapped to 16p12.1. This gene encodes the alpha chain of the interleukin-4 receptor, a type I transmembrane protein that can bind interleukin 4 and interleukin 13 to regulate IgE production. The encoded protein also can bind interleukin 4 to promote differentiation of Th2 cells. A soluble form of the encoded protein can be produced by proteolysis of the membrane-bound protein, and this soluble form can inhibit IL4-mediated cell proliferation and IL5 upregulation by T-cells. Allelic variations in this gene have been associated with atopy, a condition that can manifest itself as allergic rhinitis, sinusitus, asthma, or eczema. Polymorphisms in this gene are also associated with resistance to human immunodeficiency virus type-1 infection. Alternate splicing results in multiple transcript variants. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK2092-CAP | 96-well plate precoated with anti- human IL4R antibody | 1 |
EK2092-ST | Lyophilized recombinant human IL4R standard | 10ng/tube×2 |
EK2092-DA | Biotinylated anti- human IL4R antibody | 100μl(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex (ABC) | 100μl(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
1.96孔读板仪器。
2.自动洗板机.
3.移液器和枪头。建议使用多通道移液器/排枪。
4.Eppendorf 管。
5.额外洗板缓冲液 (neutral PBS 或 TBS。试剂盒内提供足量。如需额外缓冲液可参考如下配方)。
0.01M TBS: Add 1.2g Tris, 8.5g Nacl; 450μl of purified acetic acid or 700μl of concentrated to 1000ml H2O and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
0.01M PBS: Add 8.5g sodium chloride, 1.4g Na2HPO4 and 0.2g NaH2PO4 to 1000ml distilled water and adjust pH to 7.2-7.6. Finally, adjust the total volume to 1L.
*样品准备的相关试剂也不含在试剂盒中。Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 78 | 286 | 1170 | 81 | 312 | 1225 |
Standard deviation | 3.12 | 12.30 | 53.82 | 4.13 | 17.47 | 72.28 |
CV(%) | 4.0% | 4.3% | 4.6% | 5.1% | 5.6% | 5.9% |
Concentration(ng/ml) | 0 | 46.9 | 93.7 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.208 | 0.246 | 0.298 | 0.404 | 0.634 | 0.965 | 1.586 | 2.111 |
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