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发货周期:现货
博士德生物
cell culture supernates, serum and plasma(heparin, EDTA, citrate).
96T
货号 | EK0505 |
---|---|
产品名称 | Human P-Selectin ELISA Kit |
产品描述 | For quantitative detection of human P-Selectin in cell culture supernates, serum and plasma(heparin, EDTA, citrate). |
免疫原 | NSO, W42-A771 |
交叉反应 | There is no detectable cross-reactivity with other relevant proteins. |
敏感性 | <5pg/ml |
检测范围 | 156pg/ml-10000pg/ml |
基因名称 | SELP |
基因全名 | selectin P (granule membrane protein 140kDa, antigen CD62) |
Uniprot ID | P16109 |
指标别名 | Antigen CD62|CD62|CD62 antigen like family member P|CD62 antigen-like family member P|CD62P|FLJ45155|GMP 140|GMP-140|GMP140|Granule membrane protein 140|Granule membrane protein 140kDa|Granulocyte membrane protein|GRMP|LECAM3|Leukocyte endothelial cell adhesion molecule 3|Leukocyte-endothelial cell adhesion molecule 3|LYAM3_HUMAN|P selectin|P-selectin|PADGEM|Platelet activation dependent granule external membrane protein|Platelet activation dependent granule-external membrane protein|Platelet alpha granule membrane protein|PSEL|Selectin P|Selectin P (granule membrane protein 140kDa antigen CD62)|SELP |
背景资料 | P-selectin, also called GMP-140, CD62, or selectin P, is a 140-kD adhesion molecule, expressed at the surface of activated cells, that mediates the interaction of activated endothelial cells or platelets with leukocytes. It is stored in secretory granules and expressed at the plasma membrane after cell activation. It is known to play an important role in atherosclerosis. The major ligand for P-selectin on leukocytes is P-selectin glycoprotein ligand-1(PSGL-1). The standard product used in this kit is recombinant human P-Selectin, excluding intercellular P-Selectin and transmembrane domain. It has 730 amino acids sequence with the molecular mass of 80 KDa. |
显色时间 | 15-20分钟 |
SKU | 名称 | 数量 |
Catalog number | Description | Quantity |
EK0505-CAP | 96-well plate precoated with anti- human sP-Selectin antibody | 1 |
EK0505-ST | Lyophilized recombinant human sP-Selectin standard | 10ng/tubex2 |
EK0505-DA | Biotinylated anti- human sP-Selectin antibody | 100ul(dilution 1:100) |
AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 100ul(dilution 1:100) |
AR1106-1 | Sample diluent buffer | 30ml |
AR1106-2 | Antibody diluent buffer | 12ml |
AR1106-3 | ABC diluent buffer | 12ml |
AR1104 | TMB color developing agent | 10ml |
AR1105 | TMB stop solution | 10ml |
PLA-SEA | Adhesive cover | 4 |
Intra-Assay Precision | Inter-Assay Precision | |||||
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean(pg/ml) | 322 | 1820 | 5020 | 331 | 1849 | 5389 |
Standard deviation | 15.13 | 121.94 | 256.02 | 21.18 | 144.22 | 301.78 |
CV(%) | 4.7% | 6.7% | 5.1% | 6.4% | 7.8% | 5.6% |
Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
O.D. | 0.020 | 0.207 | 0.375 | 0.634 | 1.017 | 1.474 | 1.833 | 2.086 |
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