SP1 antibody

【求助】sp1siRNA转染细胞大家有做过的吗？

wuyf 我想看转录因子sp1对我的目的基因的表达是不是有影响，我用sp1siRNA转染细胞，发现与对照细胞相比，细胞内总RNA量减少。sp1siRNA转染细胞大家有做过的吗？ freecell 同样结果重复了几次么？做了几个剂量？每个剂量做了几个重复？ ffyy8888 :D因为转染了一些东西进去，或多或少会对细胞状态有影响的，你只要将对照做全，就可以呀，怕啥，多做几组对照

An Efficient Way of Studying ProteinProtein Interactions Involving HIF-, c-Myc, and Sp1

of mechanisms of cellular processes in biology and diseases. The hypoxia-inducible transcription factor HIF-1α possesses a non-transcriptional activity that competes with c-Myc for Sp1 binding, whereas its isoform HIF-2α lacks Sp1-binding activity

Generation of Antibody Molecules Through Antibody Engineering

been overcome to a large extent using genetic-engineering techniques to produce chimeric mouse/human and completely human antibodies. Such an approach is particularly suitable because of the domain structure of the antibody molecule ( 2 ), where functional