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文献和实验6. Quant-iT™ Protein Quantitation Kit (Cat. no. Q33210) 实验设备 5 mL Culture Tubes 实验步骤 1. Remove liver aseptically. 2. Flash-freeze liver in foil packets using liquid nitrogen. Store
Protein phosphorylation plays a pivotal role in cell cycle regulation. MPF (M-phase Promoting Factor) and MAPK (Mitogen-activated protein kinase) are two major kinases driving oocyte maturation and early embryonic divisions
These protocols should yield enough cytosol and organelles for 1-200 MT/Organelle motility assays. Solutions and Reagents • Freshly removed or flash frozen rat liver • PBS •Homogenization Buffer •Homogenization Buffer containing 0.5 mM mM
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