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BIOCHEMICALS
ATCC拥有目前全球最大的、品种最丰富的微生物、细胞系以及重组DNA原料，同时它也是科研工作者公认的提供的可靠的研究材料的供应商。
目前ATCC可以提供以下类别生物标准品：细胞株（3000种）；菌株（15000种）；动植物病毒株（2500种）；原生动物1200种以及重组物质等。
通过本公司，您可以轻松的订购到ATCC的产品，提供COA证书， 方便快捷，简化各类进口手续办理流程， 无需注册、下载和填写大量的表格资料！
广州粤晨生物科技有限公司
目前ATCC可以提供以下类别生物标准品：细胞株（3000种）；菌株（15000种）；动植物病毒株（2500种）；原生动物1200种以及重组物质等。
通过本公司，您可以轻松的订购到ATCC的产品，提供COA证书， 方便快捷，简化各类进口手续办理流程， 无需注册、下载和填写大量的表格资料！
广州粤晨生物科技有限公司
CRL-1573
293 [HEK-293] is a cell line exhibiting epithelial morphology that was isolated from the kidney of a human embryo. This cell line can be used in industrial biotechnology and toxicology research. It has applications in efficacy testing and viruscide testing.
Product category
Human cells
Organism
Homo sapiens, human
MorphologyepithelialTissuekidney; EmbryoApplications
3D cell culture
High-throughput screening
Industrial biotechnology
Toxicology
Bioproduction
Product formatFrozenStorage conditions
Vapor phase of liquid nitrogen
以下为部分产品目录：
白色念珠菌 ATCC 90029
肺炎克雷伯菌ATCC 700603
大肠艾希氏菌ATCC 35218
表皮葡萄球菌ATCC 12228
金黄葡萄球菌ATCC 29213
金黄葡萄球菌ATCC 25923
偶然分支杆菌ATCC 6841
嗜热脂肪杆菌ATCC 7953
嗜热脂肪芽孢菌ATCC 10149
流感嗜血杆菌ATCC 10211
副氏志贺氏菌ATCC 12022
堪萨斯分枝杆菌ATCC 12478
脑膜炎奈瑟菌ATCC 13090
产气夹膜梭菌ATCC 13124
胞内分枝杆菌ATCC 13950
伤沙门氏菌ATCC 14028
溶藻弧菌 ATCC 17749
副溶血性弧菌ATCC 17802
单核增生李氏特菌ATCC 19114
化脓性链球菌ATCC 19615
结合分枝杆菌ATCC 25177
脆弱拟杆菌 ATCC 25285
坏死梭杆菌 ATCC 25286
具核分枝杆菌ATCC 25586
大肠埃希氏菌ATCC 25922
金黄葡萄球菌ATCC 25923
宋氏志贺氏菌ATCC 25931
厌氧消化链球菌ATCC 27337
粪肠球菌 ATCC 29212
空肠弯曲杆菌ATCC 29428
英诺克李斯特菌ATCC 33090
大肠杆菌O157ATCC 35150
阪崎肠杆菌ATCC 51329
蜡样芽孢杆菌ATCC 11778
白色假丝酵母/白色念球菌 ATCC 10231
鲜绿青霉 ATCC 10515
桔青霉 ATCC 1109
蜡样芽孢杆菌 ATCC 11778
福氏志贺氏菌(Shigella flexneri) ATCC 12022
表皮葡萄球菌 ATCC 12228
败毒梭菌Clostridium septicum ATCC 12464
阴沟肠杆菌 ATCC 13047
产气荚膜梭菌 ATCC 13124
荧光假单胞菌 ATCC 13525
婴儿双歧杆菌 ATCC 15697
短双歧杆菌 ATCC 15700
青春双歧杆菌 ATCC 15703
恶臭假单胞菌 ATCC 17485
单增李斯特氏菌 ATCC 19111
溶组织梭菌Clostridium histolyticum ATCC 19401
生孢梭菌 ATCC 19404
破伤风杆菌/梭菌Clostridium tetani ATCC 19406
化脓性链球菌 ATCC 19615
黄杆菌属 ATCC 21047
近平滑念珠菌 ATCC 22019
变异链球菌 ATCC 25175
脆弱拟杆菌 ATCC 25285
洋葱假单胞菌 ATCC 25416
大肠埃希氏菌 ATCC 25922
宋内氏志贺氏菌(Sh.sonnei) ATCC 25931
创伤弧菌 ATCC 27562
铜绿假单胞杆菌（绿脓杆菌） ATCC 27853
粪肠球菌 ATCC 29212
两歧双歧杆菌 ATCC 29521
多形拟杆菌 ATCC 29741
粪肠球菌 ATCC 33186
空肠弯曲菌 ATCC 33291
金黄葡萄球菌 ATCC 33591
大肠埃希氏菌 ATCC 35218
屎肠球菌 ATCC 35667
迟缓真杆菌/迟缓埃格特菌 ATCC 43055
弗氏柠檬酸杆菌 ATCC 43864
淋病柰瑟氏菌 ATCC 49226
流感嗜血杆菌 ATCC 49247
肺炎链球菌 ATCC 49619
流感嗜血杆菌 ATCC 49766
粪肠球菌(高耐) ATCC 51299
阪崎肠杆菌 ATCC 51329
克柔念珠菌 ATCC 6258
枯草芽孢杆菌 ATCC 6633
肺炎克雷伯菌 ATCC 700603
热带念珠菌 ATCC 750
嗜热脂肪地芽孢杆菌 ATCC 7953
植物乳杆菌 ATCC 8014
大肠埃希氏菌 ATCC 8739
白色假丝酵母/白色念球菌 ATCC 90029
铜绿假单胞杆菌（绿脓杆菌） ATCC 9027
枯草芽孢杆菌黑色变种 ATCC 9372
艰难梭菌Clostridium difficile ATCC 9689
啤酒酵母 ATCC 9763
砖红链霉菌 ATCC19776
白色葡萄球菌 ATCC8799
皱褶青霉 ATCC 10128
酿酒酵母 ATCC 26603
酿酒酵母 ATCC 26785
运动发酵单孢菌 ATCC 29191
假单胞杆菌 ATCC 31554
粪产碱杆菌 ATCC 31555
菅囊酵母 ATCC 32691
弗氏柠檬酸杆菌 ATCC10787
荚膜红细菌 ATCC11166
发根土壤杆菌（发根植物单胞菌） ATCC11325
生孢梭菌（产芽孢梭状芽孢杆菌） ATCC11437
地衣芽孢杆菌 ATCC11946
谷氨酸棒杆菌 ATCC13032
熏衣草灰链霉菌 ATCC13306
猪霍乱沙门氏菌猪霍乱亚种 ATCC13312
脱氮假单胞菌 ATCC13867
耐放射异常球菌 ATCC13939
绿针假单胞菌 ATCC13985
肠门氏菌肠炎亚种 ATCC14028
巨大芽孢杆菌 ATCC14581
吸水链霉菌奥萨霉素亚种 ATCC15420
长双歧杆菌 ATCC15707
放射性根瘤菌 ATCC15955
短密青霉 ATCC16024
副溶血性弧菌 ATCC17802
单核增生李斯特菌 ATCC19115
缺陷短波单胞菌 ATCC19146
师岗链霉菌 ATCC19166
脱氮付球菌 ATCC19367
丝状链霉菌 ATCC19753
裂殖壶菌 ATCC20888
产朊假丝酵母 ATCC22023
乙酸钙不动杆菌 ATCC23055
珊瑚链霉菌 ATCC23901
菲律宾链霉菌 ATCC23905
里氏木霉 ATCC24449
变异链球菌 ATCC25175
沙链霉菌 ATCC25488
酪丁酸梭菌 ATCC25755
蛎灰链霉菌 ATCC27455
黄曲霉 ATCC28539
噬糖盐红菌 ATCC29252
隐甲藻 ATCC30772
干酪乳杆菌干酪亚种 ATCC334
休哈塔假丝酵母 ATCC34887
停乳链球菌 ATCC35666
昆明链霉菌 ATCC35682
扣囊内孢霉 ATCC36213
马克斯克鲁维酵母 ATCC36534
串珠镰孢 ATCC38946
米曲霉 ATCC42149
树状微杆菌 ATCC4358
潮湿纤维单胞菌 ATCC491
荧光假单胞菌 ATCC49642
水螺菌 ATCC49643
产气肠杆菌 ATCC49701
串珠镰孢 ATCC52539
金黄葡萄球菌 ATCC6538P
鼠李糖乳杆菌 ATCC7469
食油假单胞菌 ATCC8062
沙雷氏菌 ATCC8100
蛾微杆菌 ATCC8365
粪产碱菌 ATCC8750
枯草芽孢杆菌 ATCC9327
空肠弯曲杆菌ATCC 33291
产气肠杆菌ATCC13048
金黄葡萄球菌ATCC 27217
阴沟肠杆菌ATCC 13047
阪崎肠杆菌ATCC 29544
弗氏柠檬酸杆菌ATCC 43864
肺炎克雷伯氏菌ATCC 10031
小肠结肠炎耶尔森氏菌ATCC 23715
亚利桑那沙门氏菌CMCC(B) 47001
克柔假丝酵母菌ATCC 14243
光滑念珠菌ATCC 15126
创伤弧菌ATCC 27562
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文献和实验该产品被引用文献
Bacteroides clarus
DSM 22519
BACTERIA
Name:
Bacteroides clarus Watanabe et al. 2010
DSM No.:
22519, Type strain
Strain designation:
A 20, YIT 12056
Other collection no.
or WDCM no.:
or WDCM no.:
JCM 16067
Isolated from:
human faeces (healthy Japanese adult male)
Country:
Japan
Tokyo
Tokyo
Date of sampling:
09-2008
Handling procedure
Note: The cell line does not adhere to the substrate when left at room temperature for any length of time, therefore, live cultures may be received with the cells detached.The cells will re-attach to the flask over a period of several days in culture at 37°C.
To insure the highest level of viability, thaw the vial and initiate the culture as soon as possible upon receipt. If upon arrival, continued storage of the frozen culture is necessary, it should be stored in liquid nitrogen vapor phase and not at -70°C. Storage at -70°C will result in loss of viability.
- Thaw the vial by gentle agitation in a 37°C water bath. To reduce the possibility of contamination, keep the O-ring and cap out of the water. Thawing should be rapid (approximately 2 minutes).
- Remove the vial from the water bath as soon as the contents are thawed, and decontaminate by dipping in or spraying with 70% ethanol. All of the operations from this point on should be carried out under strict aseptic conditions.
- Transfer the vial contents to a centrifuge tube containing 9.0 mL complete growth medium and spin at approximately 150 to 400 x g for 8 to12 minutes.
- Resuspend cell pellet with the recommended complete growth medium (see the specific batch information for the culture recommended dilution ratio) and dispense into a 25 cm2 or a 75 cm2 culture flask. It is important to avoid excessive alkalinity of the medium during recovery of the cells. It is suggested that, prior to the addition of the vial contents, the culture vessel containing the complete growth medium be placed into the incubator for at least 15 minutes to allow the medium to reach its normal pH (7.0 to 7.6).
- Incubate the culture at 37°C in a suitable incubator. A 5% CO2 in air atmosphere is recommended if using the medium described on this product sheet.
Volumes are given for a 75 cm2 flask. Corning T-75 flasks (catalog #430641) are recommended for subculturing this product. Increase or decrease the amount of dissociation medium needed proportionally for culture vessels of other sizes.
- Remove and discard culture medium.
- Briefly rinse the cell layer with D-PBS ATCC 30-2200 to remove all traces of serum that contains trypsin inhibitor.
- Add 2.0 to 3.0 mL of Trypsin-EDTA solution ATCC 30-2101 to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal. - Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
- Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 1 x 104 to 4 x 104 viable cells/cm2 is recommended.
- Incubate cultures at 37°C.6. Subculture when cell concentration is between 6 and 7 x 104 cells/cm2.
Subcultivation Ratio: 1:6 to 1:10 weekly
Medium Renewal: Every 2 to 3 days
文献支持
JMG、DSM菌种菌株美国原装进口ATCC菌株/菌种/细胞
￥1200 - 8500
