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- Bio-Rad伯乐生命科学
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- 2025年07月09日
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文献和实验Southern Blotting:DNA Transfer
1. 8. Equilibrate blot with Buffer 3. C. Visualization 1. Prewarm CSPD Ready-to-Use to RT. 2. Transfer blot carefully from Buffer 3 (let drip ~5'') to a plastic tray. 3. In the darkroom, incubate the blot with ~25 ml
Southern Blotting: DNA Transfer
NaOH, 1.5 M NaCl (for uncharged membrane). Transfer >3 hours. 2. Crosslink if using an uncharged membrane. Solutions for Southern Hybridization and Development Hybridization 20x SSC: 3M NaCl 0.3M Na-citrate; pH7.0 Hybridization Sol'n: 5x SSC
Membrane Transfer and Crosslinking for RNA
The composition of the transfer buffer is usually a 5X SSC/10 mM NaOH solution. These mildly alkaline conditions shear the RNA into smaller fragments and denature it as it is deposited onto the membrane. A brief protocol for assembly (see Figure 1
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