EXPERION RNA LADDER

0.1-2 Kb RNA Ladder

and 0.0125% bromophenol blue) and mix well.     3) Denature the ladder in loading buffer at 70°C for 10 minutes. Keep on ice for 1-2 minutes to quench.     4) Load ladder and your RNA samples on the pre-run gel from Step 1.     5) Perform

Performance Comparison of the Experion Automated Electrophoresis System

by delivering increased sensitivity for better qualitative assessments and more reliable quantitation of RNA samples.MethodsRat brain total RNA and mRNA samples and the 6000 RNA ladder were purchased from Ambion, Inc. The Experion RNA ladder is a component

DNA LADDER检测细胞凋亡的讨论

例一 gongyulai ：我用的是药物诱导癌细胞凋亡，做DNA Ladder（用的是北京鼎国的动物细胞凋亡梯子提取试剂盒）。跑出来的电泳不是一条条带，而是每条很长的拖尾现象，Marker到是出来了。我是按说明书上0。8%的琼脂糖。我用的是60v电压。不知道什么原因？郁闷。说明书上说混合温和是什么意思，我又没用力摇。我还想问收集凋亡细胞时离心时转速多少比较好？chujun_hust ：（1）“跑出来的电泳不是一条条带，而是每条很长的拖尾现象”： 可能是由于你点样时，时间太长了，导致样品扩散