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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
Assemble all the equipment and place 200 µl of sample in one tube. Set up a second gel containing 200 µl of protein molecular weight standards. Electrophoresis is carried out at 3 mA per gel until the bromophenol blue dye reaches
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
for hESC Phosphate-buffered saline (PBS) (1X) (Ca2+ /Mg2+ -free) Equipment Centrifuge tubes (15-mL) (Falcon) Dishes (tissue culture, 10-cm) (optional; see Step 1) Filter (0.2-µm) Incubator (humidified, 37°C, 5% CO2 )
Negative Stain Electron Microscopy of Microtubules
grids just before use to increase their hydrophylicity. 2. Place sample on the grid (1-3 µl, sufficient to cover the grid surface). 3. ~10 sec later slowly pipet on 20 µl of UA stain using a P-20. While pipeting onto the grid, gently absorb
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