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文献和实验Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions Sohyun L. McElroy1 and Renee A. Reijo Pera Center for Human Embryonic Stem Cell Research and Education, Institute for Stem Cell Biology and Regenerative Medicine
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
4. In-Gel digestion with Trypsin Precool tubes with the dried gel particles and the Trypsin solution on ice Rehydrate the gel particles in the trypsin solution (12.5 ng/µl Trypsin [Promega #V5111] in 50 mM NH4HCO3) for 30-45 minutes at 4°C. Add
In-Gel Digestion of Proteins Separated by Polyacrylamide Gel Electrophoresis
particles in a Speed Vac 4. In-Gel digestion with Trypsin Precool tubes with the dried gel particles and the Trypsin solution on ice Rehydrate the gel particles in the trypsin solution (12.5 ng/µl Trypsin [Promega #V5111] in 50 mM NH
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