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文献和实验SDS Gel Electrophoresis of Tubulin\MAPs
Assemble all the equipment and place 200 µl of sample in one tube. Set up a second gel containing 200 µl of protein molecular weight standards. Electrophoresis is carried out at 3 mA per gel until the bromophenol blue dye reaches
Culturing Human Embryonic Stem Cells in Feeder-Free Conditions
for hESC Phosphate-buffered saline (PBS) (1X) (Ca2+ /Mg2+ -free) Equipment Centrifuge tubes (15-mL) (Falcon) Dishes (tissue culture, 10-cm) (optional; see Step 1) Filter (0.2-µm) Incubator (humidified, 37°C, 5% CO2 )
DNA fingerprinting - agarose gel
Restriction digests consist of: 15.75 ml ddH2 O 1 µl 10 X buffer B (Boehringer Mannheim) 0.25 µl HindIII (40 U/µl) 3 µl DNA Set up digestions in 96 well plates. Incubate at 37℃ for 4.5 hours. This can be done in a thermocycler
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