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文献和实验Immunofluorescence on ultrathin resin sections
for 10 min. Remove blocking buffer, add 25 µl of the primary antibody solution per coverslip (with a final concentration in the range of 1-5 µg specific IgG/ml) and incubate for 30 -60 min. Wash 5 times with buffer
Immunofluorescence on ultrathin resin sections
blocking buffer, add 25 µl of the primary antibody solution per coverslip (with a final concentration in the range of 1-5 µg specific IgG/ml) and incubate for 30 -60 min. Wash 5 times with buffer and incubate with fluorochrome-labelled
Mix a 5-µL aliquot of the phosphopeptides affinity-bound to the IMAC media (from Step 10) with 5 µL of 50 mM NH4HCO3 (pH 7.8) and 1 unit of calf intestine alkaline phosphatase in a 0.7-mL microcentrifuge tube. Incubate the tube on a slowly rotating
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