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- Bio-Rad伯乐生命科学
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- 2025年07月11日
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文献和实验Immunofluorescence on ultrathin resin sections
After a final wash with buffer mount coverslips on glass slides using a small drop of mounting medium (like Elvanol or Moviol 4.88) for semipermanent embedding. The addition of anti-fading agents like DABCO (25-100 mg/ml), Paraphenylenediamine (1 mg/ml) or n
Coupling Peptides To Resin For Affinity Purification
such peptides in 20% buffer, 80% DMSO. In an extreme case you can use 100% DMSO containing triethylamine. The amount of peptide to add is a question. We usually add 1-2 mg peptide/ml resin, hoping to make a resin that will bind 10-100 mgs/ml of specific antibody
Immunofluorescence on ultrathin resin sections
coverslips on glass slides using a small drop of mounting medium (like Elvanol or Moviol 4.88) for semipermanent embedding. The addition of anti-fading agents like DABCO (25-100 mg/ml), Paraphenylenediamine (1 mg/ml) or n-propyl gallate (10 mg/ml) is strongly
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