万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验GST fusion protein purification GST融合蛋白纯化
spin 2',4k on bench.10)aspirate,resuspend in 25ml HBT,spin,repeat.11)pour slurry into column (Econo 1.7x20),elute to top,then with 20 column vols.of HB-T.12)elute protein in minimal volume (5-10ml)HB 5mM GT (Sigma G4251,1.5 mg/ml).13)lN2 freeze as 100ml
GST fusion protein purification--GST融合蛋白纯化
caps,freeze in lN2,may store.9)Batch adsorb w/ 4ml 50% slurry GT-Sepharose (PL 17-0756-01),1hr,4℃ spin 2',4k on bench.10)aspirate,resuspend in 25ml HBT,spin,repeat.11)pour slurry into column (Econo 1.7x20),elute to top,then with 20 column vols.of HB-T
the RNA to the cellulose and mix on rotator for 1 hour at room temperature.6. Prepare a BIO-RAD Disposable Chromatography Column (BIO-RAD cat. #732-6008) by washing it out once with 750 l 1X NETS.7. Gently pour the RNA/cellulose mixture into the column
技术资料暂无技术资料 索取技术资料




