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文献和实验,especially for determining protein content of cell fractions and assesing protein concentrations for gel electrophoresis.Assay materials including color reagent,protein standard,and instruction booklet are available from Bio-Rad Corporation.The method
. The Bradford is recommended for general use, especially for determining protein content of cell fractions and assesing protein concentrations for gel electrophoresis. Assay materials including color reagent, protein standard, and instruction booklet
)].Under alkaline conditions,copper complexes with protein.When folin phenol reagent (phospho-molybdic-phosphotungstic reagent)is added,the Folin-phenol reagent binds to the protein.Bound reagent is slowly reduced and changes color from yellow to blue.While widely
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