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- Bio-Rad伯乐生命科学
- 美国
- 1725555
- 2025年07月08日
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文献和实验Clonality - X Chromosome Inactivation Assay
sample under study is ideal. D: PCR Set up the following PCR reaction: Cycles are as follows: E: Gel Electrophoresis Prepare gel consisting of 6% acrylamide as in LOH protocol. Add 4 µl dye to 20 µl PCR product. Denature the samples
-stop PCR only; see Steps 1-22)SSCP loading dye (for SSCP only; see Steps 23-37)95% (v/v) formamide10 mM NaOH0.25% (w/v) bromophenol blue0.25% (w/v) xylene cyanolTaq DNA polymerase (5 U/µl)TBE Buffer (1X and 5X)Prepare a 5x stock solution in 1 liter
Single tube confirmation PCR protocol
concentration of ~10 µM). We use 5 µl of each primer in 50 µl PCR reactions (~ 1 µM final primer concentration). - Label 20 thin-wall PCR tubes (5 for each isolate) and add the following primer pairs: A-B, A-kanB, C-D, kanC-D, and A-D. Tubes 1-5
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