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- Bio-Rad伯乐生命科学
- 美国
- 1703667
- 2025年07月14日
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文献和实验Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE)
The inability of conventional gel electrophoresis to separate DNA molecules exceeding 50 kb in size led to the development of pulsed-field gel electrophoresis (PFGE) by Schwartz et al, (1 )in 1982. He introduced the concept of applying
freeshotgun sequencing library from miniprep BAC DNA
precipitation (-70℃ for 30 minuets). 10.Prepare size-selected fragments Resuspend DNA pellet in 10 μl TE, pH 8.0, and add 2μl 6X DNA loading buffer, load on 1% agarose gel, side by side with Bohreenger Mannheim DNA Molecular Weight Marker VII
free shotgun sequencing library from miniprep BAC DNA
Lambda Ladder PFG Marker ( #340) on the gel. Stain with ethidium bromind and evaluate. 8.Isolate inset DNA Isolate DNA from agarose gel slice(s) depending on the insert size and with/without Not 1 cut site in the insert of individual BAC clone
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