- ￥100 - 9999
- Bio-Rad伯乐生命科学
- 美国
- 1652676
- 2025年07月12日
企业认证
相关产品推荐更多 >
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 供应商：
上海博耀商贸有限公司
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验Stable Transfection (Electroporation)
medium. Supplies & Equipment: BioRad gene pulser with capacitance extender Electroporation cuvettes with 0.4 cm interelectrode distance Reagents: sterile 1 x HeBS buffer (Hepes buffered saline) G 418 (Geneticin
ELECTROPORATION OF ES CELLS AND ISOLATION OF H/R CLONES
) + 0.73 ml PBS To A add 15 ug linearised plasmid DNA, IMMEDIATELY prior to electroporation. B no DNA 3. Using a Biorad Gene Pulser, (McMahon, A.P., and Bradley, A. (1990) Cell 62 1073-1085). "Zap" each curvette twice i.e. 500 uF 230V then 500 uF 240
Construction of Gene-Targeting Vectors by Recombineering
chemical-competent cells (Invitrogen) T4 DNA ligase and buffer (NEB) Equipment Electroporation cuvette (0.1-cm gap) (Bio-Rad) Equipment for agarose gel electrophoresis Gene Pulser Xcell Electroporation System (Bio-Rad)
技术资料暂无技术资料 索取技术资料
