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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
cOmplete?, Mini, EDTA-free Protease Inhibitor Cocktail
- 库存:
999
- 供应商:
博奥派克
- 规格:
1EA
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文献和实验A quick RNA mini-prep for Neurospora mycelial cultures
, during time series analyses or when screening transfor-mants for expression of a transformed gene. Under such circumstances, existing tech-niques are overly time consuming and yield more RNA than is necessary. The availability of a rapid RNA mini-prep
. Cocktail solutions are stored at -20 degrees and are good for approximately 6 months. I. Protease Inhibitor Cocktail The Protease inhibitor cocktail is used as 100X for extract preps and as 1000X for column elution buffers**. Cocktail
Bacterial Protein Extraction - mini scale - Sonication
optional protease inhibitors Optional additives to the lysis buffer a) 1mM PMSF or protease inhibitor cocktail 1:200 (cocktail for bacterial cells #P-8849 from Sigma) b) Dnase 100U/ml or 25-50ug/ml (SIGMA DN-25). Incubate 10min 4°C
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