- ¥900 - 3200
- 联迈生物
- LM1198M
- 中国/美国/欧洲
- 2025年07月11日
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- 详细信息
- 文献和实验
- 技术资料
- 库存：
大量
- 英文名：
PAO；Plasma Amine Oxidase
- 保质期：
2年
- 供应商：
上海联迈生物工程有限公司
- 保存条件：
RT
- 规格：
瓶
| [产品编号] | LM1198M |
| [CAS号] | 9001-66-5 |
| [规格] | BR，17Tabor u/mg |
| [包装] | 600U/3KU |
| [到货期] | 1-2天 |
英文名称：PAO；Plasma Amine Oxidase
CAS号：9001-66-5
级别：BR
来源：Bovine Plasma
Specific activity：≥17Tabor units/mg
活力定义：1 international unit oxidizes 1µmole of benzylamine per minute at 25℃, pH 7.2
催化反应：
产品描述：Bovine plasma amine oxidase has a molecular weight of 170 kDa and an optimum pH of 6.2 for spermine and 7.2 for spermidine. Amine oxidases are divided into two classes: the pyridoxal and copper containing enzyme to which plasma amine oxidase belongs, and the FAD-containing amino oxidases. Natural substrates include catecholamines, tryptamine derivatives and other physiologically active amines. Plasma amine oxidase is used in research requiring nitrogen group transfers. The molecule is composed of two identical polypeptide chains. There are two pyridoxal phosphates and two atoms of Cu+ per molecule. Bovine plasma amine oxidase is inhibited by copper chelating agents, many carboxyl reagents such as cuprizone, hydroxylamine and cyanide. Benzoic acid and benzyl alcohol are both non-competitive inhibitors (KI = 30 and 34mM respectively). The assay for determination of amine oxidases employed at Worthington is essentially that of Tabor et al., JBC, 208, 645, (1954) with the reaction temperature reduced to 25℃
性状(以下信息仅供参考)：粉末。EC3.1.1.4
用途：本品仅供科研，不得用于其它用途
保存：-20℃
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文献和实验得出的Ｘkg大鼠的用药量为Y2。 则：Y2=0.09*(Ｘ)2/3/1.722＊U＊70=3.66U(X)2/3 通过函数图像表示为： 以大鼠为例，我们认为误差在±0.1时可接受，得到如下函数：
() #交换 x 轴和 y 轴> p + ylim(0,15) #如果想截取某一段可以用此命令设置值域。> p + scale_x_continuous(breaks =seq(18,34,50) ) #设置刻度线位置> p + theme(axis.text.x = element_text(angle = 90,family = "Times",face = "italic",colour = "darkred",size=rel(0.9))) # angle = 90 设置字体角度, family
Western blotting using ECL reagent
membrane (Amersham Life Science; cat # RPN2020D)Kodak X-OMAT (XAR-5, 18X24 cm; cat # 8532665)10X TBS12.11 g Tris-base (100 mM)87.66 g NaCl (1500 mM)1 liter dd H2OAdjust pH= 7.6Washing buffer (TBS-T): 100 ml 10X TBS + 900 ml dd H2O + 1 ml Tween-20.Blocking
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