Biotin-11-dUTP, 1 mM in TE buf

ChIP Protocol-Mechanical Breakage & FA Lysis Buffer

& alternative recipe at end for lysis buffer and IP Dilution Buffer) 2. Add acid-washed glass beads, so there is a 1 to 2mm layer of liquid above the beads. 3. Vortex in the Tomy Mixer. Set it to level 10, mix 8 times for 1 minute each, and put on ice

Preparation and purification of biotin-labeled probe for ISH

solution dNTP (including biotin-11-dUTP  5 µl  plasmid or genomic DNA  1 µg

Native Chromatin Preparation and Illumina/Solexa Library Construction

19, CD36, CD56, CD123, and glycophorin A. 9. Mix well and incubate for 10 min at 4°C-8°C or on ice. 10. Add 30 µL of T cell isolation buffer per 107 cells. 11. Add 20 µL of anti-biotin microbeads (from T