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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
见包装
- 保质期：
见包装
- 英文名：
5-FAM-dUTP, 1 mM in TE buffer
- 库存：
999
- 供应商：
博奥派克
- 规格：
25 μL
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文献和实验FAM caspase 8 and 9 binding assay for embryos
into a microcentrifuge tube and add 20 µl of the 30X FAM Reagent. Mix well and protect from light!! 2) Place a 100 µl drop of the FAM Reagent onto a culture dish (we use 60 mm Petri dishes). Place embryos into the drop and incubate samples at 37 o C
ChIP Protocol-Mechanical Breakage & FA Lysis Buffer
& alternative recipe at end for lysis buffer and IP Dilution Buffer) 2. Add acid-washed glass beads, so there is a 1 to 2mm layer of liquid above the beads. 3. Vortex in the Tomy Mixer. Set it to level 10, mix 8 times for 1 minute each, and put on ice
NICK TRANSLATION OF dsDNA WITH BIOTINYLATED OR DIGOXIGENIN dUTP
solution + 27 µl bidest (1) 25 µl of each (1) +25 µl H20 = 100 µl mix 2.5 µl bio-16-dUTP (0.4 mM, ready for use) 5 µl 100 mM DTT x µl DNA (usual 1 mg disolved in 1 ml TE) 5 µl DNase I (stock
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