- ￥8260
- Cytoskeleton
- RF02-A
- 2026年06月25日
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文献和实验, sensitive, and reliable method, based on the high affinity interaction of Ras-GTP with the Ras binding domain (RBD) of Raf1, to measure the level of Ras2-GTP on total Ras2 in Saccharomyces cerevisiae . Moreover, to study the localization of Ras
GST Fusion Protein Preparation
debris at 10K 10' 4℃.Aliquot supernatant and store at -70℃.BINDING TO BEADS AND THROMBIN CLEAVAGEIncubate soluble lysate with 1/2 volume of 50% GST beads at 4℃ rocking on the Nutator for 30-60 minutes.50 ml conical Falcon tubes work well.Pour
whether the protein is insoluble. Transfer pressed lysate to a 15 ml centrifuge tube. Pellet cellular debris at 10K 10' 4C. Aliquot supernatant and store at -70C. BINDING TO BEADS AND THROMBIN CLEAVAGE Incubate soluble lysate
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