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文献和实验Determination of Histone Acetylation Status by Chromatin Immunoprecipitation
Histone acetylation is the most studied posttranslation modification of nucleosomes. Understanding the mechanisms involved in global and promoter-specific histone acetylation will shed light on the control of transcriptional regulation
Chromatin Immunoprecipitation (ChIP)
INTRODUCTION Chromatin immunoprecipitation (ChIP) is an invaluable method for studying interactions between specific proteins or modified forms of proteins and a genomic DNA region. ChIP can be used to determine whether a transcription
control). Store at -20C. Immunoprecipitation: Transfer remainder of supernatant to tube containing washed antibody conjugate. Nutate at 4?C, ≥2-4h. Wash beads 8 times with 1 ml NET-2 (ice-cold). Spin 1,000 rpm, 1 min for each wash
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