G-Actin : F-Actin In Vivo Assa

in vivo RNAi Protocols

  In vivo Purity Stealth RNAi™ siRNA and BLOCK-iT™ siRNA duplexes are specifically formulated for use in animals. Resuspend the RNA duplex in UltraPure DNase/RNase-free distilled water or appropriate DNase/RNase-free buffer (e.g. PBS

Combined 3C-ChIP-Cloning (6C) Assay: A Tool to Unravel Protein-Mediated Genome Architecture

restriction enzyme (e.g., EcoRI). Third, intramolecular ligation after cross-linking and digestion will lead to a fraction of the DNA occurring as circular DNA that cannot be cloned; this might further reduce the number of clones in such an assay. Finally

Use of In Vivo Biotinylation for Chromatin Immunoprecipitation

DNA purification kit for PCR (e.g., Qiagen): includes MiniElute columns, PBI buffer, PE buffer, and EB buffer 1.5‐ml microcentrifuge tubes