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文献和实验the ultracenrtifuge and 50.2Ti rotor to 25oC. Pellet the MTs from polymerization-incompetent tubulin by centrifugation at 100,000 x g (29,000 rpm) for 45 min at 25oC in a 50.2Ti rotor. In the cold room, discard the supernatant. Add 1ml
Porcine brain tubulin preparation
from polymerization-incompetent tubulin by centrifugation at 100,000 x g (29,000 rpm) for 45 min at 25℃ in a 50.2Ti rotor. 23. In the cold room, discard the supernatant. Add 1ml of column buffer containing 0.5 mM GTP to each tube, and being careful
Purification of Tubulin from Porcine Brain
-associated proteins require the availability of purified αβ-tubulin. In this chapter, we describe the process of purification of heterodimeric αβ-tubulin from porcine brain.
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