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文献和实验the ultracenrtifuge and 50.2Ti rotor to 25oC. Pellet the MTs from polymerization-incompetent tubulin by centrifugation at 100,000 x g (29,000 rpm) for 45 min at 25oC in a 50.2Ti rotor. In the cold room, discard the supernatant. Add 1ml
Porcine brain tubulin preparation
from polymerization-incompetent tubulin by centrifugation at 100,000 x g (29,000 rpm) for 45 min at 25℃ in a 50.2Ti rotor. 23. In the cold room, discard the supernatant. Add 1ml of column buffer containing 0.5 mM GTP to each tube, and being careful
Tubulin Polymerization with GTP/GMPCPP/Taxol
GMPCPP and add a small volume of the seeds (~1/20-1/50 vol) after the polymerization mix has been at 37¡C for 1'. Axonemes or centrosomes can also be used as nucleating structures. If the purpose is labeling or recycling the tubulin, polymerization
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