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- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
BEAKER,400ML,PLASTIC,LOW FORM,PP,6/6
- 库存:
999
- 供应商:
博奥派克
- 规格:
箱
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文献和实验Protocol for vacuum-infiltration transformation of Arabidopsis thaliana
of Agrobacterium carrying the appropriate construct. Start a 25 ml overnight (LB + antibiotics) two to three days ahead of time. Add this culture to 400ml of LB + antibiotic the day before infiltration. My experiments were done using A. tumefaciens GV3101.
SSR GEL and Silver Staining Protocol
) from Pharmacia. 2. 5U / (A260/mg) to give you mg. Add TE for a stock concentration of 400 ng/ul. a. Place in 0.5 ml tube in ice bath (small beaker) and sonicate 30 sec at 60% power (using a 3mm microtip with a 50W Vibra-Cell sonicator) b. Clean tip
Lipoprotein Isolation - First week
the refractometer . 6. Also determine UV absorbance at 280 nm. Remember to use UV-permeable plastic cuvettes (1 pair). 7. While determining the refractive index place 2 pieces of dialysis tubing (each approx. 20 cm long) into a 500 ml beaker filled
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