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文献和实验Measuring the Activity of Leucine-Rich Repeat Kinase 2: A Kinase Involved in Parkinsons Disease
Mutations in the LRRK2 (Leucine-Rich Repeat Kinase 2) gene are the most common cause of autosomal dominant Parkinson’s disease. LRRK2 has multiple functional domains including a kinase domain. The kinase activity of LRRK2 is implicated
Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions
Coimmunoprecipitation is a powerful tool to study protein-protein interactions and can be used to test for the physical association between a known protein kinase and its substrate. In this chapter, the author describes a protocol
Imaging Oscillations of Protein Kinase C Activity in Cells
The protein kinase C (PKC) family contains ten members that share a catalytic core but differ in the composition of their regulatory modules. The conventional PKCs are a subfamily whose regulatory domains bind to Ca
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