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文献和实验. In many cases, information from the environment is relayed to a central 'apoptosis rheostat' at the mitochondrion by stimulus-specific induction, modification or movement of pro-apoptotic 'BH3 domain-only' members of the Bcl-2 family (including
相关专题 细胞凋亡专题 线粒体 凋亡途径是细胞凋亡的主要途径之一,是目前研究凋亡的热点.各种凋亡刺激信号通过BH3(Bcl-2 homology domain 3)-only蛋白引起Bax(Bcl-2-asslciated protein X)蛋白移位到线粒体外膜并多聚化,形成膜通道,刺激线粒体释放细胞色素C(Cyt C) 和Smac(second mitochondrial-derived activator
Yeast Two-Hybrid Assay for Studying Protein-Protein Interactions
domain (AD) are brought together by two interacting, heterologous proteins. GAL4-yeast two-hybrid assay uses two expression vectors, one uses GAL4-DBD and the other uses GAL4-AD. DNA sequences encoding the two proteins of interest (or a protein
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