Complement Factor D

Purification, Quantification, and Functional Analysis of Complement Factor H

Complement Factor H (FH) is an abundant, non-enzymic plasma/serum glycoprotein, which has a major role in regulating activation of the complement system. It can be purified from human plasma/serum by affinity chromatography, using

2D Gel Electrophoresis（图）

of each component.2D gel electrophoresis is generally used as a component of proteomics and is the step used for the isolation of proteins for further characterisation by mass spectroscopy. In the lab this technique is used for 2 main purposes, firstly for the large

2D Gel Electrophoresis

. The higher the pH dependence of the mobility, (dµ /dpH), the better the focusing. A high electrophoretic mobility close to the IpH will efficiently transfer diffused protein molecule back to IpH. This is essentially an intrinsic factor for the protein