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- 文献和实验
- 技术资料
- 保存条件:
见包装
- 保质期:
见包装
- 英文名:
Rabbit anti BSA IgG fraction, fluorescein labeled【ASBSA-GF-FITC-1.0 mg】
- 库存:
999
- 供应商:
博奥派克
- 规格:
1.0 mg
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文献和实验-purified goat anti-mouse IgG (Jackson Labs; 1 mg/ml stock used at 1:300 dilution) Texas Red-conjugated affinity-purified donkey anti-rabbit (Jackson Labs;1 mg/ml stock used at 1:200 dilution)
Testing Rabbit Bleeds By Elisa
generated to KLH during immunization. Protocol: A. Coupling Peptide to BSA You need: 1 ml of 2 mg/ml BSA in 0.1 M NaHCO3. 1 ml of 0.2 glutaraldehyde in 0.1 M NaHCO3. 0.5 ml peptide (1 mg/ml in DMSO). Peptide can be added
antibody. For antiphosphoCREB (IgG, 0.7 mg/ml), I use 1:5,000 - 1:20,000 dilution in 4% BSA in TBST. This should be titrated for optimal results. I incubate with primary Ab 3 hours - overnight at room temp with shaking.10. Remove the antibody solution
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