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文献和实验Embryonic stem cells are pluripotent and capable of indefinite self-renewal in vitro. Human embryonic stem cells (hESC) have generally been cultivated on feeder layers of primary mouse embryonic fibroblasts (MEF) in media supplemented
Growth of Human Embryonic Stem Cells Using Derivates of Human Fibroblasts
The majority of human embryonic stem cell (hESC) lines have been derived and grown using mouse or human feeder cells, or using Matrigel�, an animal derivative rich in extracellular matrix (ECM) proteins. However, reliance on feeder layers
Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells
Conventionally, human embryonic stem cells (hESCs) are cultured on feeder cells. The most commonly used feeder cells are mouse embryonic fibroblasts. It is thought that the feeder cells provide an optimal microenvironment
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