• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Feeder-Free Growth of Undifferentiated Human Embryonic Stem Cells

        互联网

        823
        Conventionally, human embryonic stem cells (hESCs) are cultured on feeder cells. The most commonly used feeder cells are mouse embryonic fibroblasts. It is thought that the feeder cells provide an optimal microenvironment for the undifferentiated growth of hESCs by supplying currently unidentified extracellular matrix components and growth factors. Although these feeder cells are efficient, the feeder-dependent hESC culture system may not be suitable for drug screening or other research purposes that require the use of feeder-free culture methods. In this chapter, we describe in detail protocols for three widely used feeder-free systems for culturing hESCs: Matrigel-mTeSR1, CELLstart-STEMPRO hESC SFM, and vitronectin-mTeSR1.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序