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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
4℃,6个月
- 库存:
50
- 供应商:
深圳子科生物
- 规格:
100ml
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文献和实验Generation of uni-directional nested deletions in plasmid DNA
for at least 2 minutes at the desired temperature. 6) Prepare 31 sterile eppendorf tubes, each containing 3μl S1 nuclease/S1 nuclease buffer (20μl 5x S1 nuclease buffer, 79μl H2O, 1μl S1 nuclease [50U/ul]), labelled as time (t)= 0-30 minutes. Place the tubes on ice
Generation of uni-directionalnesteddeletion sin plasmid DNA
sterile eppendorf tubes, each containing 3μl S1 nuclease/S1 nuclease buffer (20μl 5x S1 nuclease buffer, 79μl H2 O, 1μl S1 nuclease [50U/ul]), labelled as time (t)= 0-30 minutes. Place the tubes on ice. 7) Remove a 2μl aliquot
Tris-HCl缓冲液,最后加重蒸水至1000ml,充分摇匀使Tris终浓度为0.05mol/L。 TBS主要用于漂洗标本,常用于免疫酶技术中。 6、Tris-TBS(PBS) 试剂: Triton X-100 10ml(1%)或3ml(0.3%) 0.5mol/L Tris缓冲液(pH7.6) 1000ml(50
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