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上海玉博生物科技有限公司
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文献和实验Preparation of Microinjection Buffer
, it is suggested that small quantities (1 gram) should be ordered and then all of it should be prepared at once. Microinjection Buffer For 50 ml: 10 mM Tris-HCl, pH 7.5 0.5 ml of 1 M Tris-HCl, pH 7.5 (autoclaved) 0.1 mM EDTA, pH 8.0 10 microliters of 0.5 M
Chromosome Conformation Capture (3C) (PROT05)
lysis buffer 10mM Tris.HCl pH 8.0 10mM NaCl 0.2% NP-40 Add protease inhibitors fresh before each lysis: 0.1mM PMSF, 1:500 protease inhibitor cocktail
Microscale Hot Borate RNA Extraction from Cotton Tissue--从棉花组织中提取微量RNA
microcentrifuge Eppendorf tubes. Add 1/10 volume (about 28 µl) of 2 M potassium acetate (pH 5.5). Incubate on ice for 15 minutes. This will removed positively-charged polysaccharides, residual proteins, and other salt-insoluble material.Centrifuge tubes at 10,000
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